Genotyping Investigation of Ciprofloxacin Resistance Genes (ParC and ParE) in Clinical Pseudomonas Aeruginosa Isolates
Keywords:
Pseudomonas aeruginosa, Fluroquinolone, Ciprofloxacin resistanceAbstract
Abstract
Pseudomonas aeruginosa appeared with burn patients is considered as one the most important opportunistic pathogen.
This study aimed to determine the mutation within genes (parC and parE) encoded for topoisomerase IV which related to
the Ciprofloxacin resistance. The distribution of P. aeruginosa among different types of specimens showed that highest
percentage, 46 (66.7%) was for burn samples. Antibiotics susceptibility showed that 34 (73.9%), 36 (78.3%), 30 (65.2%)
and 29 (63.04%) of P. aeruginosa isolates were resistant to ciprofloxacin, gentamicin, imipenem, and ceftazidime,
respectively. The gyrB gene was detected in all 21 (100%) of P. aeruginosa isolates with product size (~510 bp). The parC
and parE genes were detected in all the examined isolates. The presence of mutations in specific region (FRDR) in these
genes were screened by sending a DNA extract for genetic sequencing. The mutations in the parC and parE genes play
anessentil role in resistance to Ciprofloxacin in clinical isolates of P. aeruginosa.
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