Molecular Study of Adenovirus Associated with eyes infections in Iraq

Abstract

Abstract
The current study included the examination of 180 samples of eye swabs for ophthalmitis patients of all ages, and their
association with some demographic factors, including age, gender, clinical symptoms, recurrence of infection and chronic
diseases by means of viral transmission (VTM), which included 120 samples of patients and 60 normal samples in Iraq
From October 2020 until January 2021 . Samples were collected from patients from a consultant ophthalmologist. The
samples were classified into five groups according to the age group (1-15), (16-30), (31-45), (46-60) and (over 60) years
old. Current study involved collection and storing samples at -20°C, extracting DNA from eye samples using the Viral
DNA Kit in accordance with the manufacturer's instructions, analyzing the samples using PCR technology, and performing
an electrophoresis to analyze the results of the PCR reaction and separate the DNA from the agarose and depending on the
size of the DNA. Adenoviruses were discovered using the PCR method, and the analysis' findings indicated that there were
no adenoviruses.